IgG由四个同种型IgG1，IgG2，IgG3和IgG4组成。它包含由两条相同的50 kDaγ重（H）链和两条相同的25 kDaκ或λ轻（L）链组成的四条多肽链，它们通过链间二硫键连接。纯化的IgG可在各种免疫测定中用作参考抗原，标准品，封闭剂或包被蛋白，包括ELISA，点免疫结合，Western免疫印迹，免疫扩散，免疫沉淀和免疫电泳。其他应用包括制备免疫原和固相免疫吸附剂的原料。
IgG抗体亚型是免疫系统中最丰富的血清免疫球蛋白。它由B细胞分泌，存在于血液和细胞外液中，可防止细菌，真菌和病毒引起的感染。母体IgG通过胎盘转移到胎儿，这对新生儿抵抗感染的免疫防御至关重要。IgG介导胎盘转运并参与继发免疫反应。IgG激活补体系统的经典途径。它中和病毒颗粒和毒素。 IgG在抗体依赖性细胞介导的细胞毒性（ADCC）和吞噬作用中起关键作用。它可能与过敏有关。它具有最长的血清半衰期。
General description
IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds.[5]
Application
Purified IgG may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA,[1] dot immunobinding, Western immunoblotting, immunodiffusion, immunoprecipitation[2] and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid-phase immunoadsorbents.
包装
10, 50, 100 mg in poly bottle
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.[3]
IgG mediates transplacental transport and participates in the secondary immune response.[6] IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody dependent cell-mediated cytotoxicity (ADCC) and phagocytosis.[5] It might be linked to allergy.[4] It has the longest serum half life.[6]
Analysis Note
Protein concentration should be determined after reconstitution by absorbance at 280 nm (E1%/280 = 14).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
性质
| Quality Level | 200 |
| biological source | sheep |
| antibody form | purified immunoglobulin |
| grade | reagent grade |
| assay | ≥95% (SDS-PAGE) |
| form | essentially salt-free, lyophilized powder |
| isotype | IgG |
| solubility | saline: 1 mg/mL at -20 °C (Repeated freezing and thawing is not recommended.) |
| conjugate | unconjugated |
| storage temp. | 2-8°C |
安全信息
RIDADR NONH for all modes of transport
京公网安备 11010902000808号 | 京ICP备11047009号-1