TE缓冲液，Tris-EDTA buffer solution；BioUltra, for molecular biology, pH 8.0

图片 TE缓冲液，Tris-EDTA buffer solution；BioUltra, for molecular biology, pH 8.0
Tris-EDTA缓冲液；TE buffer solution；MDL编号: MFCD00236359 eCl@ss: 32129211 PubChem化学物质编号: 329770217 NACRES: NA.25
制造商品牌: 西格玛 Sigma-Aldrich
货号（SKU）: 93283
MDL号: MFCD00236359
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¥264.37

说明

应用

Tris-EDTA buffer solution has been used as a component in Tris-EDTA (TE) buffer to resuspend DNA pellets for total DNA extraction from herbage samples and single-stranded carrier DNA extraction from salmon sperm.
 

生化/生理作用

Ethylenediaminetetraacetic acid (EDTA) and its salts are used in a variety of medical and pharmaceutical applications. It is a calcium chelator that also acts as an anticoagulant and a detoxicant in the case of heavy metal poisoning. EDTA is frequently used in analytical chemistry for complexometric titrations and several other applications. It is used as a food additive to bind metal ions due to its low toxicity.
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer.
 

特点和优势

  • DNases, RNases, phosphatases, and protease-free
  • Insoluble matter, passes filter test

其他说明

该缓冲剂为 10mM Tris-HCl, 1mM EDTA 二钠，pH 8.0。
 
 

属性

等级

for molecular biology

质量水平

200

产品线

BioUltra

形式

liquid

杂质

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH值（酸碱度）

8.0±0.2 (25 °C)

痕量阳离子

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

紫外吸收

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.01

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

 

安全信息

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 20 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 20
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