RPMI-1640培养基，RPMI-1640 Medium；With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

 

设计用于细胞培养应用中氨基酸稳定同位素标记。
补充 0.100 g/L L -精氨酸- ¹³ C ₆ 、 ¹⁵ N ₄ HCl（产品 608033）、0.100 g/L L -亮氨酸- ¹³ C ₆ 、 ¹⁵ N（产品 608068）和 0.100 g/L L -赖氨酸- ¹³ C ₆ 、 ¹⁵ N ₂ （产品 608041）。