DEAE-Sepharose ® 用于亲和层析、蛋白层析和离子交换层析。DEAE-Sepharose ® 已被用于研究人类疾病的发病机制和开发检测艰难梭菌致病菌株毒素的新方法 。 参数DEAE Sepharose CL-6BAutoclavableIn 0.1 M NaCl at 121°C for 30 min Avoid UsingOxidizing materials Binding Capacity/ml Chromatography Medium:Thyroglobulin (MW 669 000) 2 mg/mL, HSA (MW 68 000) 170 mg/mL, alpha-lactalbumin (MW 14 300) 150 mg/mL Chemical stabilityAqueous buffers, 1.0 M acetic acid, 1.0 M NaOH, 8 M Urea, 8 M guanidine hydrochloride, ethanol, methanol Flow velocity< 150 cm/h Ion Exchanger TypeWeak anion Ionic Capacity0.13-0.17 mmol/ml LigandDiethylaminoethyl Matrix6% Cross-linked agarose Particle Size45 μm-165 μm Particle size, d50V 1~90 μm Storage4 to 30°C, 20% Ethanol pH stability, CIP 22–14 pH stability, operational 33–9 1Median particle size of the cumulative volume distribution.2pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.3pH range where resin can be operated without significant change in function.