Capto™ Butyl-S is the least hydrophobic resin in the Capto™ HIC resin range. It combines high capacity, high flow rate, and low back pressure.
Capto™ Butyl-S is a hydrophobic interaction chromatography resin designed for the capture and intermediate stages of protein purification. It is the least hydrophobic resin in the range of high-productivity Capto™ HIC resins.
- High-productivity resin designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations
- Improved productivity and process economy in downstream operations compared with predecessor Butyl-S Sepharose™ resin
- Excellent chemical stability
- The least hydrophobic resin in the range of high-productivity Capto™ HIC resins
- Low risk of denaturation of relatively strong hydrophobic solutes
Capto™ Butyl-S high-productivity HIC resin
Capto™ Butyl-S是一种定制设计的填料，专为大规模纯化mAb、mAb变体和其他生物分子而开发。它是高生产率Capto™ HIC树脂系列中疏水性最低的树脂。
该填料设计用于在相对较低的盐浓度下结合和洗脱相对较强的疏水性分子。在分离过程的初始阶段，它特别有用，可以在没有严格要求的情况下去除大量杂质（例如，调节pH值、盐浓度等）。
它是一种 BioProcess™填料，可满足大型生物制药制造商对高效且具有成本效益的蛋白质纯化的需求。该树脂包括经过验证的制造方法、有保障的供应和监管支持文件 （RSF），以协助工艺验证和提交给监管机构。
Capto™ Butyl-S is a custom-designed resin developed for large-scale purification of mAbs, mAb variants, and other biomolecules. It is the least hydrophobic resin in the range of high-productivity Capto™ HIC resins.
This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations. It is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g., adjustment of pH, salt concentration, etc.).
It is a BioProcess™ resin that meets the demands of large-scale biopharmaceutical manufacturers for efficient and cost-effective protein purification. The resin includes validated manufacturing methods, secured supply, and Regulatory Support File (RSF) to assist process validation and submission to regulatory authorities.
Other Capto™ HIC resins
We offer other Capto™ resins for hydrophobic interaction chromatography with different hydrophobicities (Fig 1).
High-flow and high-capacity HIC resins:
High-resolution HIC resins:
See an overview of our HIC resins.
| 参数 | Capto™ Butyl-S |
|---|
| Ligand | 1-butylmercaptan |
| Ligand Concentration | 10.0 - 13.5 µmol/mL resin |
| Matrix | Highly cross-linked agarose, spherical |
| Particle Size 1 | ~75 µm |
| Pressure/Flow Specification 2 | ≥ 600 cm/h at < 0.3 MPa in a 1 m diameter column and 20 cm bed height (at 20°C using process buffers with the same viscosity as water) |
| pH stability, CIP 3 | 2-14 |
| pH stability, operational 4 | 3-13 |
| Storage solution | 20% ethanol |
