羟乙基哌嗪乙硫磺酸，HEPES；reagent grade, ≥99% (titration), crystalline

HEPES has been described as one of the best all-purpose buffers available for biological research.[7] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[1]

 

HEPES has been used:

• As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo†
• As a component of Hanks′ solution used in the degradation testing of Iron†
• As a component of HEPES medium in the transfection of cell culture with plasmids†
• Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells†
• As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies†