结晶紫 [龙胆紫]，Crystal Violet；for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

图片 结晶紫 [龙胆紫]，Crystal Violet；for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)
别名：六甲副蔷薇苯胺氯化物；甲基紫 10B；碱性紫 3；龙胆紫；甲基紫10B；甲紫；六甲基紫；六甲基玫苯胺盐酸 盐；盐基紫3；Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

线性分子式: C25H30N3Cl CAS号: 548-62-9 分子量: 407.98 颜色索引号: 42555 Beilstein: 3580948 MDL编号: MFCD00011750 PubChem化学物质编号: 329759365 NACRES: MA.02
制造商品牌: 西格玛 Sigma-Aldrich
货号（SKU）: 61135
CAS号: 548-62-9
MDL号: MFCD00011750
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¥419.25

说明

结晶紫用作革兰氏染色的活性成分，主要染色剂，用于革兰氏阴性菌与革兰氏阳性菌的分化。它是具有亲脂性阳离子的六-N-甲基化三氨基三苯甲烷。它具有略微非平面的共轭系统。
结晶紫通常用于革兰氏染色及其变体；并染色淀粉样蛋白，细菌成分和维管植物组织。它也用于植物染色体的染色。结晶紫也适用于琼脂糖凝胶中的DNA可视化。该染料仅在高浓度DNA存在下使用。结晶紫还有助于细胞活力的比色测量。

一般描述

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It is a hexa-N-methylated triaminotriphenylmethane with a lipophilic cation. It possesses a slightly nonplanar conjugated system.
 

应用

Crystal violet is commonly used in the Gram stain and its variants; and to stain amyloid, bacterial components, and vascular plant tissues. It is also used for the staining of plant chromosomes.Crystal violet is also suitable for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal Violet also helps in the colorimetric measurement of cell viability.
 
 

属性

等级

for microscopy (Bact., Bot., Hist., Vit.)
indicator (pH 0.1-2.0)

质量水平

200

形式

powder

visual transition interval

0.1-2.0, yellow-green to blue-violet

mp

205 °C (dec.) (lit.)

密度

1.190 g/cm3

ε (extinction coefficient)

≥1750 at 585-595  nm in water

适用性

suitable for microscopy (Bact., Bot., Hist., Vit.)

抗生素抗菌谱

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

作用机制

cell membrane | interferes
enzyme | inhibits

储存温度

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

 

安全信息

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 50 | 欧洲Eur. ≥ 12 | 東京Tokyo ≥ 20 | 香港与北京 ≥ 50
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