碱性品红 [基本品红]，Basic Fuchsin；for microscopy Certistain®, Dye, ≥80% spectrophotometric assay

图片 碱性品红 [基本品红]，Basic Fuchsin；for microscopy Certistain®, Dye, ≥80% spectrophotometric assay
图片 碱性品红 [基本品红]，Basic Fuchsin；for microscopy Certistain®, Dye, ≥80% spectrophotometric assay
图片 碱性品红 [基本品红]，Basic Fuchsin；for microscopy Certistain®, Dye, ≥80% spectrophotometric assay
别名：基本紫罗兰 14, 苯胺，4-[（4-氨基苯基）（4-亚氨基-2，5-环己二烯-1-亚基）甲基]-2-甲基 盐酸盐, 苯胺，4-[（4-氨基苯基）（4-亚氨基-2，5-环己二烯-1-亚基）甲基]-2-甲基；Basic Violet 14, Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2-methyl hydrochloride, Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2-methyl

CAS号 632-99-5 经验分子式（希尔表示法） C20H20ClN3 分子量 337.85
制造商品牌: 西格玛 Sigma-Aldrich
货号（SKU）: 1.15937
CAS号: 632-99-5
MDL号: MFCD00012569
签订合同 √ 正规发票 √ 技术支持 √ 质量保障 √ 全程可追溯 √
¥760.00

说明

一般描述

This staining dye Fuchsin (C.I. 42510) - for microscopy Certistain® is used for staining of acid-fast bacteria (AFB) according to Kinyoun and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung.
The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. As a measure to enhance the absorption of the fuchsin dye and thus the formation of the mycolate-fuchsin complex in the cell wall, the carbolfuchsin solution applied to the specimen is normally heated to evaporation point. If the Kinyoun carbolfuchsin solution is used, the heating of the solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided.
Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In the present operating instructions is counterstained accordance with methylene blue.
Pretreatment of the specimens with Sputofluol™ dissolves the bacteria from the surrounding viscid sputum and cell material. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
 

分析说明

染料含量（分光光度法）： ≥ 80 %
鉴别（紫外/可见光谱）：通过测试
最大吸收λ 最大值（乙醇 50 %）：549 - 552 nm
比吸光系数 A 1%/1cm（λmax; 0.003 g/l；乙醇 50 %）。2150 - 2710
TLC-测试：通过测试
干燥失重（100 °C）： ≤ 15 %
适用于显微镜检查：通过测试
 

法律信息

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany
 
 

属性

质量水平

400

形式

solid

组成

Dye, ≥80% spectrophotometric assay

pH值（酸碱度）

5-6 (25 °C, 1 g/L in H2O)

mp

235 °C (decomposition)

溶解性

4 g/L

堆积密度

500 kg/m3

εmax

2150-2710 at 549-552 nm in 50% ethanol at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

储存温度

2-30°C

InChI

1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,23H,21-22H2,1H3;1H

InChI key

AXDJCCTWPBKUKL-UHFFFAOYSA-N

 

安全信息

警示用语：

Danger

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Irrit. 2 - Skin Irrit. 2

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 10 | 欧洲Eur. ≥ 8 | 東京Tokyo ≥ 6 | 香港与北京 ≥ 60
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