Methyltetrazine-NHS ester has an
N-hydroxysuccinimide (NHS) ester that can be reacted with primary amines and a methyltetrazine, which is reactive to
trans-cyclooctenes.
[1][2] The NHS ester reacts with primary amines (e.g. side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic pH to form covalent bonds. The short spacer arm adds minimal mass to modified molecules (230.2 daltons). It is generally used in click chemistry applications in bioconjugate chemistry.
[1][2][3][4]