异柠檬酸脱氢酶1来源于人，Isocitrate Dehydrogenase 1 (NADP+) human [IDH1]；recombinant, expressed in E. coli, lyophilized powder, ≥80 units/mg protein

Isocitrate dehydrogenase 1 (IDH1) gene is located on human chromosome 2q33. IDH1 is present in the cytoplasm and peroxisomes.[1] It is expressed at high levels in the mammalian liver. IDH1 consists of a C-terminal tripeptide peroxisome, that targets the signal one sequence.[2]

 

Isocitrate dehydrogenase 1 (IDH1) help in the oxidative carboxylation of isocitrate to α-ketoglutarate, that results in the formation of reduced nicotinamide adenine dinucleotide phosphate (NADPH).[1] It participates in lipid metabolism and glucose sensing. Suppressing IDH1 in pancreatic islets affects the insulin secretion, induced by glucose and leads to increase in lactate production.[2]

 

One unit corresponds to the amount of enzyme which converts 1.0 μmol DL-isocitrate to α-ketoglutarate per minute at pH 7.4 and 37 °C (NADP as cofactor).

 

Supplied as a lyophilized powder containing Tris-HCl pH 8.0, trehalose, ammonium sulfate and DTT