台盼蓝,Trypan Blue;Dye content 60%

别名:台酚蓝;直接蓝 14, Direct blue 14,锥蓝;锥虫蓝;曲利本蓝;

线性分子式: C34H24N6O14S4Na4 CAS号: 72-57-1 分子量: 960.81 颜色索引号: 23850 Beilstein: 4360496 EC 号: 200-786-7 MDL编号: MFCD00003969 PubChem化学物质编号: 24858268 NACRES: NA.47
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): 302643
CAS号: 72-57-1
MDL号: MFCD00003969
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¥436.00

说明

一般描述

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.
 

应用

台盼蓝已被用作台盼蓝排斥试验/细胞活力试验中的染料,以检测死细胞。[1][2]
 

生化/生理作用

台盼蓝是一种蓝色的酸性染料,含有两个偶氮生色团。是一种大的亲水性四磺酰化阴离子染料。也可用于屏幕植物提取物的细胞毒性属性。[3]此外,台盼蓝用于玻璃体切除术中的视网膜前膜染色。[4]
Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:
  • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
  • to stain plant tissue in order to visualize cell death.

 

属性

形式

powder

质量水平

组成

Dye content, 60%

mp

>300 °C (lit.)

溶解性

H2O: 1 mg/mL

λmax

607 nm

ε (extinction coefficient)

≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

human ... PTPN1(5770) , PTPRF(5792)

 

安全信息

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Carc. 1B

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 50 | 欧洲Eur. ≥ 12 | 東京Tokyo ≥ 20 | 香港与北京 ≥ 50
商品标签 [tags]