邻苯二甲醛，Phthaldialdehyde [OPA]；for fluorescence, ≥99.0% (HPLC)

图片 邻苯二甲醛，Phthaldialdehyde [OPA]；for fluorescence, ≥99.0% (HPLC)
别名：1,2-苯二甲醛，OPA，邻苯二醛，酞醛，o-Phthalaldehyde，o-Phthalic dicarboxaldehyde，Benzene-1,2-dicarboxaldehyde

Empirical Formula (Hill Notation): C8H6O2 CAS号: 643-79-8 分子量: 134.13 Beilstein: 878317 EC 号: 211-402-2 MDL编号: MFCD00003335 PubChem化学物质编号: 57652816 NACRES: NA.32
制造商品牌: 西格玛 Sigma-Aldrich
货号（SKU）: 79760
CAS号: 643-79-8
MDL号: MFCD00003335
签订合同 √ 正规发票 √ 技术支持 √ 质量保障 √ 全程可追溯 √
¥2,470.10

说明

苯二甲醛，也称为O-苯甲醛或OPA，是五列（HPLC）衍生物试剂。 OPA与初级氨基酸（AA）反应，除胱氨酸以形成高度荧光加合物。 分别以345nm和455nm的激发和发射波长监测衍生物的荧光。 HPLC技术有效地分离了OPA-AA衍生物。
苯二甲醛或OPA决定了动物组织和食物样品中蛋白质中的氨基酸。 乳酸细菌产生的酪胺和苯甲胺的氨基酸加合物的定量使用OPA作为HPLC的柱前衍生物试剂。 谷胱甘肽（GSH/GSSG）的细胞内形式使用O-苯甲醛作为殖民前荧光剂进行了研究。
苯二甲醛可用于氨基酸的柱前衍生化，用于高性能液相色谱（HPLC）分离。 它也可用于蛋白质硫醇基团的流式细胞仪测量。

一般描述

Phthaldialdehyde, also known as o-Phthalaldehyde or OPA, is a pre-column (HPLC) derivatization reagent. OPA reacts with primary amino acids (AA) except cystine to form highly fluorescent adducts. The fluorescence of the derivatives is monitored at excitation and emission wavelengths of 345nm and 455nm, respectively. The HPLC technique efficiently separates the OPA-AA derivatives.
 

应用

Phthaldialdehyde or OPA determines amino acids in proteins from animal tissue and food samples. Quantification of amino acids adducts of tyramine and phenylethylamine produced by lactic acid bacteria used OPA as the pre-column derivative reagent for HPLC. Intracellular forms of glutathione (GSH/GSSG) are investigated using o-Phthalaldehyde as the pre-column fluorescent agent.
 

生化/生理作用

Phthaldialdehyde can be used for the pre-column derivatization of amino acids for high-performance liquid chromatography (HPLC) separation. It may also be used for flow cytometric measurements of protein thiol groups.
 
 

属性

等级

for fluorescence

质量水平

100

测定

≥99.0% (HPLC)

形式

solid

杂质

≤0.5% phthalic acid

mp

54-56 °C

荧光

λex 334 nm; λem 455 nm (Thiol Adduct)
λex 340 nm; λem 450 nm in reaction buffer (with glycine)

储存温度

2-8°C

SMILES string

O=Cc1ccccc1C=O

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H

InChI key

ZWLUXSQADUDCSB-UHFFFAOYSA-N

 

安全信息

警示用语：

Danger

危险分类

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(F)

269.6 °F - closed cup

闪点(C)

132 °C - closed cup

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 50 | 欧洲Eur. ≥ 12 | 東京Tokyo ≥ 20 | 香港与北京 ≥ 50
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