Superdex 75 FF size exclusion chromatography resin offers very high resolution fractionation for separation, polishing and product formulation of recombinant proteins.
Superdex 75 Fast Flow是Superdex 75的高流速定制版；Superdex是一种超敏反应凝胶过滤填料，颗粒均匀、刚性好、不收缩，在150cm/h流速下有极高的分辨率，Superdex 75 FF的分离范围：3,000~80,000。
Application
Superdex® 系列也称琼葡糖凝胶、葡琼糖凝胶、葡琼凝胶，是将葡聚糖凝胶基质的高选择性与交联琼脂糖凝胶的化学和物理稳定性相结合，可用于蛋白质层析、凝胶过滤层析、凝胶过滤介质、树脂和分离介质。现已被用于从食用菌猴头菇中纯化和表征一种新型漆酶。Superdex还被用于紫荆种子作为潜在人类肝细胞癌抑制剂的纯化和机理研究。针对尺寸排阻色谱法的高分辨率制备分离进行了优化，列出了球状蛋白和葡聚糖的推荐分馏范围。Superdex 75 prep grade size exclusion chromatography resin offers very high resolution fractionation for separation, polishing and product formulation of recombinant proteins.
- Size exclusion chromatography resin providing exceptional resolution with short run times and good recovery.
- Superdex 75 prep grade is recommended for purification of recombinant proteins.
- BioProcess resin supported for industrial applications and well-established in approved processes.
Superdex prep grade is a preparative size exclusion chromatography resin with a composite matrix of dextran and agarose. This matrix combines the excellent size exclusion chromatography properties of cross-linked dextran (Sephadex) with the physical and chemical stabilities of highly cross-linked agarose, to produce a separation resin with outstanding selectivity and high resolution. In addition, its low non-specific interaction permits high recovery of biological material. Together these properties make Superdex prep grade the first choice size exclusion chromatography resin for all applications from laboratory to process scale.
Superdex prep grade is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As a BioProcess resin, Superdex prep grade meets industrial demands with security of supply and comprehensive technical and regulatory support.
性质
| Parameter | Superdex 75 Fast Flow |
|---|
| BioProcess resin | Yes |
| Particle size, d50V1 | ~34 µm |
| Matrix | Composite of cross-linked agarose and dextran |
| Particle Size | 24 µm-44 µm |
| pH stability, operational2 | 3–12 |
| pH stability, CIP | 1–14 |
| Fractionation range (Mr) (globular proteins) | 3 × 10³–8 × 10⁴ |
| Fractionation (Mp) Dextrans | 5 x 10²-3 x 10⁴ |
| Storage | 4 to 30°C, 0.2 M sodium acetate in 20% Ethanol |
| Chemical stability | Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile |
| Certificate of Analysis | Yes |
