葡萄糖-6-磷酸脱氢酶来源于肠系膜明串珠菌,Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides [G-6-P-DH, G6PDH];recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

别名:6-磷酸葡糖脱氢酶;Zwischenferment;6-磷酸葡萄糖脱氢酶;葡萄糖6磷酸脱氢酶

CAS号: 9001-40-5 EC 号: 1.1.1.49 (BRENDA, IUBMB) EC 号: 232-602-6 MDL编号: MFCD00081656 NACRES: NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): G8529
CAS号: 9001-40-5
MDL号: MFCD00081656
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¥696.60

说明

应用

Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used:
  • in the coupled spectrophotometric assay for PGI (F6P to G6P reaction)
  • to study the starch time course using Nicotiana tabacum leaves
  • to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings
  • to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings

生化/生理作用

作为戊糖磷酸途径的第一步,葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯。
Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene.
 

单位定义

在 NAD 存在下,在pH 7.8,30°C 下,1 个单位将每分钟氧化 1.0 μmol D-6-磷酸葡萄糖至 6-磷酸-D-葡萄糖酸盐。
 

外形

含有Ficoll和Tris 缓冲盐的冻干粉
 
 

属性

重组

expressed in E. coli

质量水平

形式

lyophilized powder

specific activity

≥550 units/mg protein (biuret)

组成

Protein, 10-40% biuret

application(s)

agriculture

异质活性

creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%

储存温度

2-8°C

 

安全信息

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 20 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 20