葡聚糖凝胶G75,Sephadex G-75

Sephadex G75是一种性能良好的凝胶过滤填料,用于对分子量 > 80000 的大生物分子进行脱盐和更换缓冲液。17005001;17005003;17-0050-01;17-0050-03
货号(SKU): 17005001
CAS号: 37224-29-6
MDL号: MFCD00132772
签订合同 √ 正规发票 √ 技术支持 √ 质量保障 √ 全程可追溯 √
¥1,950.00

Sephadex G-75

Sephadex G-75 is well established gel filtration resin for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

葡聚糖凝胶G-75是一种性能良好的凝胶过滤填料,用于对分子量 > 80000 的大生物分子进行脱盐和更换缓冲液。

  • 一步即可快速脱盐,去除污染物并转移到新的缓冲液中。
  • 适用于大生物分子(分子量 > 80000)的缓冲液更换和纯化
  • Sephadex G 范围内的最高排阻限度 80000
  • 经典凝胶过滤填料。

Sephadex 是通过将葡聚糖与表氯醇交联制备的凝胶过滤填料。不同类型的 Sephadex 的交联程度不同,因此其溶胀度和分子分离范围也不同。

 

参数Sephadex G-75
可高温高压灭菌湿润状态(pH 值 7.0),120°C 下 30 min。 
排阻限度 (Mr)(球状蛋白)> 8 x 10⁴ 
Fractionation range (Mr) globular proteins 1   3 × 10³–8 × 10⁴ 
基质交联右旋糖酐 
PSDdry (within specification) 240 µm ~ 120 µm 
PSDwet 390–280 µm 
推荐的最大操作流速 490 cm/h in a BPG 100/500 column with 10 cm inner diameter and 20 cm bed height (at room temperature using water) 
储存4 至 30°C 
Swelling of dry resin 519 mL/g 
pH 稳定性(操作) 62–10 
  • 1Separation expected between Kd 0.2-0.8.
  • 2Within sieving limits, guide for which Sephadex resin to choose depending on application. ≥ 80% of the volume of particles are found within the range shown
  • 3Calculated wet PSD range based on water regain. Range within which particles can be found is presented within parenthesis and should be used as guide for choosing filter
  • 4The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
  • 5Swelling in swollen bed in 0,02M NaCl, should be used as guide to column/vessel size. Representative swelling presented, statistical foundation not available
  • 6填料可在功能无显著变化的情况下调控的 pH 值范围。
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 50 | 欧洲Eur. ≥ 12 | 東京Tokyo ≥ 20 | 香港与北京 ≥ 50
商品标签 [tags]