葡聚糖凝胶G100超细粒，Sephadex G-100 Superfine

图片 葡聚糖凝胶G100超细粒，Sephadex G-100 Superfine
Sephadex G100是通过将葡聚糖与表氯醇交联制备的凝胶过滤填料。17006101；17006102；17-0061-01；17-0061-02
制造商品牌: 思拓凡 Cytiva , (安)法玛西亚 Amersham Pharmacia
货号（SKU）: 17006101
CAS号: 9050-94-6
MDL号: MFCD00132235
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¥3,550.00

Sephadex G-100 Superfine

Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, resin, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and resin are are preferred for large scale group separations where high flow rates and low operating pressures are required.

  • 一步即可快速脱盐，去除污染物并转移到新的缓冲液中。
  • 经典凝胶过滤填料。

葡聚糖凝胶G-100超细粒是通过将葡聚糖与表氯醇交联制备的凝胶过滤填料。不同类型的 Sephadex 的交联程度不同，因此其溶胀度和分子分离范围也不同。有五种不同的 G 类型，包括用于小分子的 G-10 再到用于大分子的 G-75，Sephadex G-10 是其中之一。Sephadex G-50 有 4 种不同粒径（粗粒、填料、细粒和超细粒），Superfine 的粒径最小，可通过较短扩散距离实现更高效的分离。对于需要高流速和低操作压力的大规模基团分离，优选粗粒填料。

 

参数Sephadex G-100 Superfine
可高温高压灭菌湿润状态（pH 值 7.0），120°C 下 30 min。 
排阻限度 (Mr)（球状蛋白）> 1 x 10⁵ 
Fractionation range (Mr) globular proteins 1   4 × 10³ – 1 × 10⁵ 
基质交联右旋糖酐 
PSDdry (within specification) 210 µm ~ 40 µm 
PSDwet 325–100 µm 
推荐的最大操作流速Care must be taken to avoid compressing the gel. Do not increase the pressure beyond 0.096 bar. 
Swelling of dry resin 421 mL/g 
pH 稳定性（操作） 52–10 
  • 1Separation expected between Kd 0.2-0.8.
  • 2Within sieving limits, guide for which Sephadex resin to choose depending on application. ≥ 80% of the volume of particles are found within the range shown
  • 3Calculated wet PSD range based on water regain. Range within which particles can be found is presented within parenthesis and should be used as guide for choosing filter
  • 4Swelling in swollen bed in 0,02M NaCl, should be used as guide to column/vessel size. Representative swelling presented, statistical foundation not available
  • 5填料可在功能无显著变化的情况下调控的 pH 值范围。
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