Sephadex G-100
Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, resin, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and resin are are preferred for large scale group separations where high flow rates and low operating pressures are required.
- 一步即可快速脱盐，去除污染物并转移到新的缓冲液中。
- 经典凝胶过滤填料。
葡聚糖凝胶G-100是通过将葡聚糖与表氯醇交联制备的凝胶过滤填料。不同类型的 Sephadex 的交联程度不同，因此其溶胀度和分子分离范围也不同。有五种不同的 G 类型，包括用于小分子的 G-10 再到用于大分子的 G-75，Sephadex G-10 是其中之一。Sephadex G-50 有 4 种不同粒径（粗粒、填料、细粒和超细粒），Superfine 的粒径最小，可通过较短扩散距离实现更高效的分离。对于需要高流速和低操作压力的大规模基团分离，优选粗粒填料。
| 参数 | Sephadex G-100 |
|---|
| 可高温高压灭菌 | 湿润状态（pH 值 7.0），120°C 下 30 min。 |
| 排阻限度 (Mr)（球状蛋白） | > 1 x 10⁵ |
| Fractionation range (Mr) globular proteins 1 | 4 × 10³ – 1.5 × 10⁵ |
| 基质 | 交联右旋糖酐 |
| PSDdry (within specification) 2 | 40 µm ~ 120 µm |
| PSDwet 3 | 100–310 µm |
| 推荐的最大操作流速 | Care must be taken to avoid compressing the gel. Do not increase the pressure beyond 0.096 bar. |
| Swelling of dry resin 4 | 21 mL/g |
| pH 稳定性（操作） 5 | 2–10 |
