硫酸博来霉素 [硫酸博莱霉素]，Bleomycin sulfate from Streptomyces verticillus [BLM]；for fluorescence, mixture of bleomycin sulfate salts, lyophilized, powder or crystals, white to off-white

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  • 硫酸博来霉素 [硫酸博莱霉素]，Bleomycin sulfate from Streptomyces verticillus [BLM]；for fluorescence, mixture of bleomycin sulfate salts, lyophilized, powder or crystals, white to off-white
图片 硫酸博来霉素 [硫酸博莱霉素]，Bleomycin sulfate from Streptomyces verticillus [BLM]；for fluorescence, mixture of bleomycin sulfate salts, lyophilized, powder or crystals, white to off-white
别名："博莱霉素"同"博来霉素"；硫酸博来霉素来源于轮枝链霉菌；博来霉素硫酸盐；博莱霉素硫酸盐；Blenoxane；Bleo；Blexane

CAS号: 9041-93-4 EC 号: 232-925-2 MDL编号: MFCD00070310 PubChem化学物质编号: 57647391 NACRES: NA.85
制造商品牌: 西格玛 Sigma-Aldrich
货号（SKU）: 15361
CAS号: 9041-93-4
MDL号: MFCD00070310
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¥3,350.00

说明

化学结构：糖肽
硫酸博莱霉素用于研究某些细胞系中的肿瘤特异性细胞毒性活性，例如人类正常口腔细胞（牙龈纤维瘤HGF、牙髓细胞HPC和牙周膜成纤维细胞HPLF）、人类口腔鳞状细胞癌（HSC-2、HSC-3、HSC-4、Ca9-22和NA）和人类早幼粒细胞白血病HL-60细胞。它用于研究DNA链断裂/断裂的自由基介导机制，并作为转化的细胞选择剂，尤其是植物转化体。建议用作10-100μg/ml的选择剂。
硫酸博莱霉素与DNA结合，导致特定碱基序列的ssDNA断裂，并抑制DNA合成。这种抑制作用需要博来霉素结合氧和金属离子。它还可以在较小程度上但更选择性地切割RNA。它作为细胞凋亡的诱导剂和调节剂，并抑制肿瘤血管生成。

一般描述

Chemical structure: glycopeptide
 

应用

Bleomycin sulfate is used to study tumor-specific cytotoxic activity in certain cell lines such as human normal oral cells(gingival fibrobast HGF, pulp cell HPC and periodontal ligament fibroblast HPLF), human oral squamous cell carcinoma (HSC-2, HSC-3, HSC-4, Ca9-22 and NA) and human promyelocytic leukemia HL-60 cells. It is used to study free radical-mediated mechanisms of DNA strand scission/breaking and as a transformed cell selection agent, especially for plant transformants. Recommended for use as a selection agent at 10-100 μg/ml.
 

生化/生理作用

Bleomycin sulfate binds to DNA, causes ssDNA scission at specific base sequences and inhibits DNA synthesis. This inhibitory action requires bleomycin to bind oxygen and a metal ion. It can also cleave RNA, to a lesser degree but more selectively. It acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis.
 

组分

This product is a mixture of glycopeptides antibiotics containing Bleomycin A2 (70%) and B2(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.
 

注意

This product should be stored at 2-8°C.
 

制备说明

The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.
 

其他说明

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
 
 

属性

生物来源

Streptomyces verticillus

质量水平

200

等级

for fluorescence

形式

powder or crystals

质量

lyophilized

颜色

white to off-white

溶解性

H2O: 20 mg/mL, clear, colorless

痕量阳离子

Cu: ≤0.1%

抗生素抗菌谱

Gram-negative bacteria
Gram-positive bacteria
neoplastics

作用机制

DNA synthesis | interferes

储存温度

2-8°C

SMILES string

[O-]S([O-])(=O)=O.C[C@@H](O)[C@@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@H](NC(=O)c1nc(nc(N)c1C)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]2O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]2O[C@@H]3O[C@H](CO)[C@@H](O)[C@@H](OC(N)=O)[C@@H]3O)c4c[nH]cn4)C(=O)NCCc5nc(cs5)-c6ncc(s6)C(=O)NCCC[S+](C)C.C[C@@H](O)[C@@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@H](NC(=O)c7nc(nc(N)c7C)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]8O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@@H]9O[C@H](CO)[C@@H](O)[C@@H](OC(N)=O)[C@@H]9O)c%10c[nH]cn%10)C(=O)NCCc%11nc(cs%11)-c%12ncc(s%12)C(=O)NCCC[S+](C)C

InChI

1S/2C55H83N17O21S3.H2O4S/c2*1-20-33(69-46(72-44(20)58)25(12-31(57)76)64-13-24(56)45(59)82)50(86)71-35(41(26-14-61-19-66-26)91-54-43(39(80)37(78)28(16-73)90-54)92-53-40(81)42(93-55(60)88)38(79)29(17-74)89-53)51(87)67-22(3)36(77)21(2)47(83)70-34(23(4)75)49(85)63-10-8-32-68-27(18-94-32)52-65-15-30(95-52)48(84)62-9-7-11-96(5)6;1-5(2,3)4/h2*14-15,18-19,21-25,28-29,34-43,53-54,64,73-75,77-81H,7-13,16-17,56H2,1-6H3,(H13-,57,58,59,60,61,62,63,66,67,69,70,71,72,76,82,83,84,85,86,87,88);(H2,1,2,3,4)/t2*21-,22+,23+,24-,25-,28-,29+,34+,35+,36-,37+,38+,39-,40-,41-,42+,43-,53-,54-;/m00./s1

InChI key

OOXTWFJZZAJGKA-CNLAFNBISA-N

 

安全信息

象形图

Health hazard
GHS08

警示用语：

Danger

危险分类

Carc. 2 - Muta. 1B - Repr. 2

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 50 | 欧洲Eur. ≥ 12 | 東京Tokyo ≥ 20 | 香港与北京 ≥ 50
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