琼脂粉，Agar；Type E, suitable for plant cell culture

Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked β-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.[1]

Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally, it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked ß-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.[1]

 

Agar has been used as reagent for the following studies:

• Plant cell culture studies.
• As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.[2]
• For the solidification of culture medium of cork oak somatic embryos.[3]
• to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants[4]

Agar suspension culture may be used for the analysis of cell transformation of palyoma virus.[5] It might have been used for incubation of single antibody-forming cell to investigate the plaque formation.[6]

Agar has been used to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants.[4]