溶壁酶来源于藤黄节杆菌，Lyticase from Arthrobacter luteus；lyophilized powder, ≥200 units/mg solid

Lyticase from Arthrobacter luteus has been used:

• as a component in spheroplasting buffer to prepare yeast nuclear extracts[1]
• for digestion in sample preparation for β-glucan enzymatic assay using yeast[1]
• to lyse cells for RNA isolation[2]

Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation.[3] Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities.[4] It is useful with yeast cells such as e.g. Candida,[5] Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.

溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。

 

一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA₈₀₀ 。

 

更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer