异硫氰酸胍水溶液,Guanidine thiocyanate solution;BioUltra, for molecular biology, ~6 M in H2O

别名:硫氰酸亚脲,硫氰酸胍,Guanidinium rhodanide, Guanidinium thiocyanate

线性分子式: NH2C(=NH)NH2 · HSCN CAS号: 593-84-0 分子量: 118.16 Beilstein: 3563461 MDL编号: MFCD00013027 PubChem化学物质编号: 57651292 NACRES: NA.25
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): 50983
CAS号: 593-84-0
MDL号: MFCD00013027
签订合同 √ 正规发票 √ 技术支持 √ 质量保障 √ 全程可追溯 √
¥1,406.56

说明

硫氰酸胍是一种具有强蛋白质变性作用的离液剂。它广泛用于RNA分离方案。硫氰酸胍可以不可逆地灭活RNase和DNase,并破坏细胞膜。它通过干扰蛋白质的二级结构来溶解蛋白质。硫氰酸胍还将核蛋白与核酸分离,并用蛋白酶帮助蛋白质的消化。它提高了蛋白酶K的活性,从而使其成为首选蛋白酶。

一般描述

Guanidine thiocyanate is a chaotropic agent that functions as a strong protein denaturant. It is widely used in RNA isolation protocols. Guanidine thiocyanate can irreversibly inactivate RNase and DNase and breaks cellular membranes. It dissolves proteins by disturbing their secondary structures. Guanidine thiocyanate also separates nucleoproteins from nucleic acids and aids the digestion of the proteins with proteases. It improves the activity of proteinase K thus making it the protease of choice.
 

应用

Guanidine thiocyanate solution has been used to extract:
  • plasma RNA from human immunodeficiency virus (HIV)-infected and control patients
  • total RNA from collected tissues (shoots, flowers, fruits, and seeds)
  • in reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA from teratocarcinoma cell line (Tera-1)

注意

稍微加热至40-50℃,可能结晶的产物会再次溶解。
 
 

属性

等级

for molecular biology

质量水平

产品线

BioUltra

浓度

~6 M in H2O

杂质

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

折射率

n20/D 1.474-1.483

pH值(酸碱度)

4.5-7.0 (25 °C)

痕量阴离子

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤500 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

吸光度

cut-off at 280 nm

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

 

安全信息

象形图

CorrosionExclamation mark

警示用语:

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

补充剂危害

储存分类代码

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 10 | 欧洲Eur. ≥ 8 | 東京Tokyo ≥ 6 | 香港与北京 ≥ 60
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