尿酸酶来源于念珠菌属，Uricase from Candida sp.；recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

图片 尿酸酶来源于念珠菌属，Uricase from Candida sp.；recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid
别名：尿酸氧化酶 ，环拉酸，脲酸酶，尿酸盐：氧还原酶，Urate: oxygen oxoreductase，uricase，urate oxidase

CAS号: 9002-12-4 EC 号: 1.7.3.3 (BRENDA, IUBMB) MDL编号: MFCD00082126 PubChem化学物质编号: 24900619 NACRES: NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号（SKU）: U0880
CAS号: 9002-12-4
MDL号: MFCD00082126
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¥1,718.14

说明

尿酸参与嘌呤分解代谢。它催化高度不溶性尿酸转化为5-羟基异尿酸盐。尿酸累积会导致肝/肾损害或慢性痛风。在小鼠中，编码尿酸酶的基因突变导致尿酸突然升高。缺乏该基因的小鼠表现出高尿酸血症、高尿酸症和尿酸结晶性梗阻性肾病。

一般描述

缺乏尿酸酶的小鼠出现尿酸肾病和尿崩症，重组尿酸酶治疗可缓解。 [1]
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.
 

应用

Uricase from Candida sp. has been used:
  • to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
  • to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
  • for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

生化/生理作用

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.
 

单位定义

在 25°C、pH 8.5 的条件下，1 个单位每分钟将把 1.0 μmol 尿酸氧化成尿囊素。
 

外形

粉末；含柠檬酸盐和蔗糖
 
 

属性

重组

expressed in E. coli

质量水平

200

形式

lyophilized powder

specific activity

≥2 units/mg solid

分子量

35 kDa by SDS-PAGE

储存温度

−20°C

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

 

安全信息

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

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储存温度 Storage temp.-20°C低温冷冻
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