IgA抗体是分泌性抗体，在胃肠道，呼吸道和泌尿生殖道，眼泪，初乳和唾液的粘膜中大量存在。 因此，分泌型IgA有助于抵抗粘膜表面病原体的体液防御机制。人IgA是通过分级分离和离子交换分级分离而从合并的正常人初乳中纯化得到的。
纯化的IgA可用作各种免疫测定（包括ELISA，斑点免疫结合，免疫印迹，免疫扩散和免疫电泳）的参考抗原，标准品，封闭剂或包被蛋白。 其他应用包括制备免疫原和固相免疫吸附剂的原料。 来自人初乳的IgA在ELISA（1 mg / ml）和凝胶电泳中用作标准。
General description
IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears, colostrum and saliva. Thus secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces[2]
Human IgA is purified from pooled normal human colostrum by fractionation and ion-exchange fractionation.
Application
Purified IgA may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA, dot immunobinding, immunoblotting, immunodiffusion and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solidphase immunoadsorbents. IgA from human colostrum was used as a standard in ELISA (1 mg/ml)[1][3] and gel electrophoresis.[4]
包装
5, 25 mg in glass bottle
Reconstitution
Reconstitute with buffered saline (150 mM sodium chloride).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
性质
| Quality Level | 200 |
| grade | reagent grade |
| assay | ≥95% |
| form | essentially salt-free, lyophilized powder |
| conjugate | unconjugated |
| storage temp. | 2-8°C |
安全信息
RIDADR NONH for all modes of transport
